Emu Bay locals that keep an eye on nesting hooded plovers were distressed to witness two chicks run over on Sunday morning.
Mia Vickery is urging locals and visitors to give the nesting area an extra wide berth and to slow down and be on the lookout for the small shorebirds.
Plover advocates would like to see seasonal closure east of the creek to protect the threatened species.
"Bunting and signs have been up for the last month while the hoodies have been on their nest," she said.
"The chicks hatched on Monday so adults and chicks are now feeding so often seen closer to waters edge. This is probably the riskiest time for them.
"Cars do not slow down. It would be good to have a seasonal closure east of the creek to at least give the hoodies that breed here a chance."
The Kangaroo Island Council at its October meeting voted to approve new beach access signs at the Island's beaches.
The updated signs will make it clear that driving is only permitted 200 metres either side of the access point for the purpose of launching boats.
Emu Bay beach however is the only beach were driving is permitted its entire length.
BirdLife Australia meanwhile will hold two free hooded plover workshops at Penneshaw and Parndana on October 25 and 26. Please RSVP to email@example.com or call 0422 798 388.
A report into the 2018 Hooded Plover Biennial Count on Kangaroo Island was being finalised with results expected soon.
Beach-nesting Birds Project coordinator Renée Mead said Kangaroo Island was the first location in South Australia and Victoria to have chicks hatch this season.